Two of the officers were evacuated by helicopter. The third was expected to be transported by road before also being airlifted, the JCS said in a statement, without providing further details on their condition.
South Korean media reported the blast might have been caused by a mine.
The explosion took place south of the Military Demarcation Line within the DMZ, the JCS said. The heavily fortified border has separated the countries since the 1950-1953 Korean War ended with an armistice rather than a peace treaty.
Mine explosions and other blasts are a recurring hazard in and around the DMZ, where legacy devices and suspected stray mines have periodically injured military personnel on both sides.
North Korean forces resumed fortification work starting in March near the border, including mine-laying and fence construction, media reports said.
Earlier this month, South Korean lawmaker Yu Yong-weon said minefields and other installations built by North Korea crossed the Military Demarcation Line by roughly 10 to 15 metres (33-49 ft) at six locations in the western area of the DMZ.
(Reporting by Joyce LeeEditing by Ed Davies)

A view of a North Korean flag from the Tongilchon Village People near the Demilitarised Zone (DMZ) in Paju, Gyeonggi-do province, South Korea, 25 December 2025. According to South Korea's Joint Chiefs of Staff (JCS), North Korea launched a test firing of new anti-air missiles toward the East Sea on 24 December. EPA/JEON HEON-KYUN
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