The East African country confirmed its first cases of the disease, a viral hemorrhagic fever that can cause death, among some patients, in mid-September.
It reported 66 confirmed cases with 15 deaths and 51 recoveries, the health ministry said on Friday.
"It has been a long journey but today, here come to the end of Marburg outbreak in Rwanda. So, Marburg is over according to World Health Organization guidelines," Health Minister Sabin Nsanzimana told a news conference.
"It took us for 42 days since the last patient tested negative and discharged... Last night at midnight exactly, was the end of the 42nd day therefore we declare Marburg over in Rwanda."
(Reporting by Philbert Girinema; Writing by George ObulutsaEditing by Bate Felix)

Rwanda managed to contain an outbreak of one of the world's most feared high-consequence pathogens, the Marburg virus. (Image: Johns Hopkins Bloomberg School of Public Health / Wikipedia)
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