The 58-key piano, on which actor and singer Dooley Wilson performed "As Time Goes By," has a pre-sale estimate of $800,000 to $1.2 million for the Dec. 14 auction.
Wilson sang the song while playing the role of Sam in the 1942 film as Humphrey Bogart and Ingrid Bergman, the star-crossed lovers Rick and Isla, leaned on the piano in a flashback to their time in Paris.
"How can anything say 'I love you' better than the piano from Casablanca?" David Redden, vice chairman and director of the special projects department at Sotheby's, said in a statement announcing the sale.
The film, set in Morocco during World War Two, won three Academy Awards including best picture, best writing and best director for Michael Curtiz.
Watch: Play it again, Sam
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